RESUMO
An aquifer located within a petroleum processing plant in Moin, Costa Rica, suffers hydrocarbon pollution. This study aimed to determine the ability of indigenous microorganisms from this site to degrade polycyclic aromatic hydrocarbons [PAHs] to evaluate the feasibility of an eventual bioremediation process. Aerobic conditions were found in the aquifer, while microbial analyses of the groundwater indicated the presence of important hydrocarbon-degrading populations. Sixteen PAH-degrading strains were isolated with the ability to grow on naphthalene [5 strains], phenanthrene [3], fluorene [6] and pyrene [2]. Most of the identified isolates belonged to the genus Pseudomonas, although, Comamonas, Sphingomonas Stenotrophomonas and Delftia were also found. A mixture of selected strains was evaluated by its performance of PAH degradation in soil-slurry systems, where efficiency of removal was naphthalene > fluorene > phenanthrene > pyrene. This study is an initial approach to evaluate the feasibility of applying a bioremediation process in the contaminated site